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A box-model of carrying capacity of the Thau lagoon in the context of ecological status regulations and sustainable shellfish cultures ArchiMer
Pete, Romain; Guyondet, Thomas; Bec, Beatrice; Derolez, Valerie; Cesmat, Ludovic; Lagarde, Franck; Pouvreau, Stephane; Fiandrino, Annie; Richard, Marion.
The decrease of microbial and nutrient inputs from the watershed has long dominated lagoon ecosystem management objectives. Phytoplankton biomass and abundance have drastically decreased for more than a decade and Zostera meadow have gradually recovered, expressing lagoon ecosystem restoration such as Thau lagoon. Do the progressive achievement of the good ecological status of the Thau lagoon possibly threatens the shellfish industry in terms of production and oyster quality, by reducing the carrying capacity? To provide answers about the right balance to be achieved between conservation and exploitation, a new numerical tool was developed to help in decision-making. We hereby propose to incorporate a Dynamic Energy Budget type shellfish production model...
Tipo: Text Palavras-chave: Shellfish farming; Carrying capacity; Stakeholder implication; Nitrogen and phosphorus inputs; Water frame directive; Ecological indicators; Phytoplankton depletion; Coupled biogeochemical-deb model.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00622/73395/75312.pdf
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Application of the coastal ecosystem complex concept toward integrated management for sustainable coastal fisheries under oligotrophication ArchiMer
Hori, Masakazu; Hamaoka, Hideki; Hirota, Masahito; Lagarde, Franck; Vaz, Sandrine; Hamaguchi, Masami; Hori, Juri; Makino, Mitsutaku.
Harmonizing coastal fisheries with water-quality improvement has become an essential factor for the sustainable use of coastal ecosystem services. Here, we present the scope of our study based on an interdisciplinary approach including ecological actions, socio-economic actions and socio-psychological actions. We chose to focus on the interaction between oyster aquaculture and seagrass vegetation as a typical ecological action using the coastal ecosystem complex (CEC) concept. Coastal organisms have adapted their traits to the environment over a long period of time, so that restoration of the CEC represents reconstruction of the original process of coastal production. Subtidal seagrass vegetation with intertidal oyster reefs is the original CEC in Japan,...
Tipo: Text Palavras-chave: Oyster aquaculture; Seagrass; Indigenous and local knowledge; Integrated coastal management.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00429/54019/57439.pdf
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Associations between farming practices and disease mortality of Pacific oyster Crassostrea gigas in a Mediterranean lagoon ArchiMer
Pernet, Fabrice; Lagarde, Franck; Le Gall, Patrik; Roque D'Orbcastel, Emmanuelle.
We present the first large-scale, high-resolution spatial and temporal pattern of disease mortality caused by Ostreid herpesvirus 1 (OsHV-1) that has affected Pacific oyster Crassostrea gigas in France every year since 2008, in relation to local farm use. Mortality was monitored in healthy oyster spat deployed at 106 locations in the Thau Mediterranean lagoon during the epizootic that occurred in spring 2011. We conducted a field survey of farming practices in the areas where the sentinel oysters were placed before the epizootic. We found that the risk of disease mortality in sentinel oysters was higher within the bivalve farming area than outside it, suggesting that the infection pressure is much higher in areas with intensive farming activity. The risk...
Tipo: Text Palavras-chave: Aquaculture; Epidemiology; OsHV-1; Shellfish farming.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00201/31248/30662.pdf
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Bilan de l'année 1996 : Cultures d'huîtres en eaux profondes ; Suivi de l'activité physiologique de C. gigas sur le banc de Ronce (mortalités anormales sur le banc de Ronce) ; Valorisation des claires ostréicoles ArchiMer
Goulletquer, Philippe; Le Moine, Olivier; Soletchnik, Patrick; Razet, Daniel; Turpin, Vincent; Robert, Jean-michel; Gras, Paul; Faury, Nicole; Geairon, Philippe; Taillade, Sylvie; Lodato, Marie; Lagarde, Franck.
La contractualisation SRC/IFREMER-URAPC concernait en 1996 trois actions spécifiques : • Expertise pour les cultures d'huîtres en eaux profondes, • Suivi de l'activité physiologique sur le banc de Ronce, • Valorisation des claires ostréicoles - optimisation du processus d'affinage. Le premier thème n'a pu être abordé que via les différentes réunions préparatoires SRC Ré Centre Ouest, Marennes-Oléron et les commissions des cultures marines. Depuis l'accord de la CCM de La Rochelle en date du 11/04/1996, le dossier reste en attente d'un accord budgétaire des différents partenaires. Le second thème concerne le suivi de l'activité physiologique des huîtres creuses sur le banc ostréicole de Ronce-Perquis. La méthodologie est basée sur l'utilisation d'un...
Tipo: Text Palavras-chave: Ostréiculture; Elevage en eaux profondes; Crassostrea gigas; Physilogie; Mortalité; Claire ostréicole; Bassin de Marennes Oléron; Charente-Maritime.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00102/21316/18936.pdf
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Budget énergétique de l'huître perlière, Pinctada margaritifera, dans le lagon de Takapoto ArchiMer
Pouvreau, Stéphane; Tiapari, Jérôme; Gangnery, Aline; Garnier, M.; Lagarde, Franck; Robert, Stéphane; Jonquieres, Gerard; Teissier, Hinano; Prou, Jean; Bennett, Auguste; Caisey, Xavier; Haumani, Gaby; Buestel, Dominique; Bodoy, Alain.
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Ano: 1999 URL: http://archimer.ifremer.fr/doc/00041/15176/12513.pdf
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Caractérisation des sites du canal de Port La Nouvelle pouvant recevoir les courantomètres H-ADCP, Channel Master RD instrument ArchiMer
Lagarde, Franck; Fiandrino, Annie; Canessa, Julien; Lefeuvre, Patrick.
CONTEXTE - Après avoir prospecté durant l'année 2004 pour définir des sites intéressants pour la mesure de champs de courant et l'estimation de débits dans le Canal de Port La Nouvelle avec des courantomètres horizontaux H-ADCP Channel Master (RD Instrument), 6 sites ont été retenus (mars 2005). Chaque site retenu doit être caractérisé par sa bathymétrie et une observation des champs de courant sur l'ensemble de la section de chenal (mesure tridimensionnelle de champs de courant) en vue d'une estimation ponctuelle du débit de chenal. Etant donné la sensibilité des H-ADCP Channel Master (RD Instrument), des essais ont été réalisés sur les sites retenus afin d'observer le comportement des appareils et leur aptitude à mesurer les courants en fonction des...
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Ano: 2005 URL: http://archimer.ifremer.fr/doc/00074/18489/16032.pdf
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Changes in planktonic microbial components in interaction with juvenile oysters during a mortality episode in the Thau lagoon (France) ArchiMer
Richard, Marion; Bec, Beatrice; Vanhuysse, Charles; Mas, Sébastien; Parin, David; Chantalat, Camila; Le Gall, Patrik; Fiandrino, Annie; Lagarde, Franck; Mortreux, Serge; Ouisse, Vincent; Rolland, Jean-luc; Degut, Anaïs; Hatey, Elise; Fortune, Martine; Roque D'Orbcastel, Emmanuelle; Messiaen, Gregory; Munaron, Dominique; Callier, Myriam; Oheix, Jocelyne; Derolez, Valerie; Mostajir, Behzad.
Oysters modify the planktonic microbial community structure by their filtration and NH4 excretion activities. While many studies have been conducted on this subject with adult oysters, none had been carried out in situ with juveniles. Pacific oyster juveniles (Magallana gigas, previously Crassostrea gigas) died massively all over the world since 2008 in relation with OsHV-1 infection. During mortality episodes, sick and dead oysters are not separated from healthy live ones, and left to decay in the surrounding environment, with unknown consequences for the nutrient cycle and planktonic microbial components (PMC). The present study aimed to elucidate for the first time the interactions between oyster juveniles and PMC during a mortality episode. Innovative...
Tipo: Text Palavras-chave: Magallana gigas; Crassostrea gigas; Spat; OsHV-1 mu Var; Mortality; Picoplankton; Heterotrophic flagellates; Ciliates; Uronema; Balanion.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00474/58559/61115.pdf
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Déterminisme du recrutement larvaire de l’huître creuse (Crassostrea gigas) dans la lagune de Thau. ArchiMer
Lagarde, Franck; Fiandrino, Annie; Richard, Marion; Bernard, Ismael; Mortreux, Serge; Ubertini, Martin; Chiantella, Claude; Boj, Marie; Meddah, Slem; Leurion, Axel; Soriano, Solenn; Fuhrmann, Marine; Le Gall, Patrik; Chiera, Caroline; Berteaux, Tom; Guillou, Jean-louis; Roque D'Orbcastel, Emmanuelle; Perignon, Adeline; Bonnet, Delphine; Roques, Cecile; Bec, Beatrice; Cochet, Helene; Gervasoni, Erika; Miron, Gilles; Pouvreau, Stephane.
Since spat mortality events that were firstly detected in 2008, Mediterranean shellfish industry seeks to exploit the Pacific oyster spat from lagoon natural recruitment. The CRCM, Cepralmar and Ifremer developed accordingly a research project named PRONAMED Phase 1 and 2 - 2010-2014, in order to define the economic potential of spatfall and improve knowledge of the reproductive cycle of Crassostrea gigas in this specific environmental context. The results of the past three year‘s study (2012, 2013, 2014) showed that broodstock have the ability to provide larvae after sexual maturation and spawning. A coincidence was noted between moon phase (new and full moon) and spawnings with an amplifying effect of storms on the intensity of spawning (Ubertini et al....
Tipo: Text Palavras-chave: Lagune; Recrutement; Naissain; Huître creuse; Ecologie larvaire; Cycle de reproduction; Captage naturel.; Lagoon; Recruitment; Spat; Pacific oysters; Larval ecology; Reproduction Cycle; Natural spat collection..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00279/39054/37620.pdf
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Développement d’une méthode d’estimation des débits dans les graus de lagunes. Application sur la lagune de Bages-Sigean ArchiMer
Fiandrino, Annie; Lagarde, Franck; Le Gall, Patrik; Messiaen, Gregory; Chiantella, Claude; Roucher, Benoit; Meyer, Johann.
Les travaux présentés dans le présent rapport finalisent l’étude engagée dans le cadre de l’Axe 1 : « Amélioration des connaissances et compréhension des processus concernant le fonctionnement et le devenir du littoral méditerranéen » de la Convention Cadre entre l’Ifremer et l’Agence de l’Eau Rhône Méditerranée et Corse portant sur le « Développement de modèle hydrodynamiques et hydrosédimentaires sur les masses d’eau de transition ». Au terme de cette étude, nous disposons d’une méthode de jaugeage des débits en canal, opérationnelle dans la majorité des configurations de profils verticaux de courant rencontrées. Le chenal portuaire de Port-La-Nouvelle, unique voie de communication entre la lagune de Bages-Sigean et la Méditerranée, a été retenu comme...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00060/17104/14620.pdf
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Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome ArchiMer
De Lorgeril, Julien; Petton, Bruno; Lucasson, Aude; Perez, Valérie; Stenger, Pierre-louis; Degremont, Lionel; Montagnani, Caroline; Escoubas, Jean Michel; Haffner, Philippe; Allienne, Jean-françois; Leroy, Marc; Lagarde, Franck; Vidal Dupiol, Jeremie; Gueguen, Yannick; Mitta, Guillaume.
Background: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein...
Tipo: Text Palavras-chave: Pacific oyster; Oyster disease; Resistance; OsHV-1; Antiviral molecular pathways; Invertebrate immunity.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00602/71369/69811.pdf
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Duality of trophic supply and hydrodynamic connectivity drives spatial patterns of Pacific oyster recruitment ArchiMer
Lagarde, Franck; Fiandrino, Annie; Ubertini, Martin; Roque D'Orbcastel, Emmanuelle; Mortreux, Serge; Chiantella, Claude; Bec, Beatrice; Bonnet, Delphine; Roques, Cécile; Bernard, Ismael; Richard, Marion; Guyondet, Thomas; Pouvreau, Stephane; Lett, Christophe.
The recent discovery of Pacific oyster Crassostrea gigas (also known as Magallana gigas) spatfields in a Mediterranean lagoon intensely exploited for shellfish farming (Thau lagoon) revealed significant contrasts in spatial patterns of recruitment. We evaluated the processes that drive spatial patterns in oyster recruitment by comparing observed recruitment, simulated hydrodynamic connectivity and ecological variables. We hypothesized that spatial variability of recruitment depends on (1) hydrodynamic connectivity and (2) the ecology of the larval supply, settlement, metamorphosis, survival and biotic environmental parameters. We assessed recruitment at 6-8 experimental sites by larval sampling and spat collection inside and outside oyster farming areas...
Tipo: Text Palavras-chave: Crassostrea gigas; Coastal lagoon; Larval ecology; Spatial patterns; Connectivity; Settlement; Recruitment; Oligotrophication.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00598/70981/69271.pdf
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Écologie de la reproduction de l’huître Crassostrea gigas en lagune méditerranéenne ArchiMer
Lagarde, Franck.
The Mediterranean lagoons host many anthropogenic uses including shellfish aquaculture since the beginning of the 20th century. Imported in 1970, the cupped oyster, Crassostrea gigas, has adapted perfectly and has allowed an important development of oyster’s industry until 2008, the year of occurence of spat outbreak related to the emergence of an Ostreid Herpes-virus μvar. The scarcity of spat and the speculation on this product changed regionally cultural practices and the supply of juveniles, with in particular the wish of developing a local activity of spat collection. However the cycle of reproduction of the species and the recruitment of juveniles were poorly known in lagoon environments. In this context, the objective of this thesis is to...
Tipo: Text Palavras-chave: Huître; Écologie larvaire; Ressource trophique; Connectivité; Recrutement; Lagune côtière; Oyster; Larval ecology; Trophic resource; Connectivity; Recruitment; Coastal lagoon.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00472/58347/60901.pdf
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Effect of environmental drivers on the reproduction of the pacific oyster Crassostrea gigas in the Mediterranean: The case of the Thau lagoon ArchiMer
Ubertini, Martin; Lagarde, Franck; Le Gall, Patrik; Mortreux, Serge; Pernet, Fabrice; Fiandrino, Annie; Pouvreau, Stephane; Roque D'Orbcastel, Emmanuelle.
The aquaculture of the oyster Crassostrea gigas is largely based on natural recruitment of the species on spat collectors along the french Atlantic coast, the remaining spat being provided by hatcheries and nurseries. Due to an increase in the climate/meteorological variability, during the last twenty years, the natural spat collection has become variable, from null to overabundant depending on the year. Since 2008, the oyster spat mortalities worsen the situation, reducing the available spat and increasing the cost of the latter. However, the temporal variability of the natural recruitment modalities in the Mediterranean do not allow the aquaculture industry to rely on native spats, making the understanding of natural recruitment in the Mediterranean a...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00248/35894/34422.pdf
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Eléments d'écophysiologie d'huîtres Crassostrea gigas issues du bassin de Marennes-Oléron ArchiMer
Goulletquer, Philippe; Lagarde, Franck.
Le bassin de Marennes-Oléron est le premier site ostréicole européen. Depuis 1988 des mortalités chroniques apparaissent sur les élevages conchylicoles. En 1996, un programme de suivi de mortalité et des paramètres environnementaux a été développé. Les différentes conclusions retenues au cours des années antérieures font état de brusques variations de température suspectées dans l'apparition de ces mortalités. Une approche écophysiologique des huîtres par l'intermédiaire de la mesure de respiration a permis de déterminer l'effet significatif d'un stress thermique (∆T=28°C). La maturité sexuelle apparaît également comme un facteur prépondérant dans la demande métabolique. A l'issue de cette étude, il apparaît que les huîtres matures et de surcroît stressées...
Tipo: Text Palavras-chave: Ecophysiologie; Physiologie; Huître creuse; Bassin de Marennes-Oléron.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00076/18761/16332.pdf
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Evaluation of the lake model FLake over a coastal lagoon during the THAUMEX field campaign ArchiMer
Le Moigne, Patrick; Legain, Dominique; Lagarde, Franck; Potes, Miguel; Tzanos, Diane; Moulin, Eric; Barrie, Joel; Salgado, Rui; Messiaen, Gregory; Fiandrino, Annie; Donier, Sylvie; Traulle, Olivier; Costa, Maria Joao.
The THAUMEX measurement campaign, carried out during the summer of 2011 in Thau, a coastal lagoon in southern France, focused on episodes of marine breezes. During the campaign, three intensive observation periods (IOPs) were conducted and a large amount of data were collected. Subsequently, standalone modelling using the FLake lake model was used, first to assess the surface temperature and the surface energy balance, and second to determine the energy budget of the water column at the measurement site. Surface fluxes were validated against in situ measurements, and it was determined that heat exchanges are dominated by evaporation. We also demonstrated that the model was sensitive to the light extinction coefficient at Thau, due to its shallowness and...
Tipo: Text Palavras-chave: Lake modelling; Surface energy budget; Coastal breeze; Water heat storage; Surface temperature.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00161/27257/25466.pdf
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Facteurs explicatifs de la dynamique spatio-temporelle des mortalités d’huîtres creuses Crassostrea gigas dans la lagune de Thau ArchiMer
Pernet, Fabrice; Lagarde, Franck; Cesmat, Ludovic; Jeannée, Nicolas; Barret, Jean; Le Gall, Patrik; Quere, Claudie; Fiandrino, Annie; Miguet, Marine; Boulet, Hélène; Keck, Nicolas; Roque D'Orbcastel, Emmanuelle.
Cette étude avait pour objectif principal de déterminer les facteurs explicatifs de la dynamique spatio-temporelle des surmortalités d’huîtres creuses dans la lagune de Thau. Les facteurs étudiés étaient : la pathologie (détection d’ADN appartenant à l’Ostreid herpesvirus 1 OsHV-1 et au groupe bactérien Vibrio splendidus), l’état énergétique des huîtres (triglycérides, sucres totaux et acides gras comme marqueurs trophiques), les pratiques d’élevage et l’hydrodynamique. Afin de répondre à ces objectifs, nous avons suivi la mortalité d’un lot d’huîtres sentinelles, âgé de moins d’un an, maintenu indemne de mortalité, dans lequel on ne détectait pas d’ADN appartenant à OsHV-1. Le lot a été déployé mi-mars 2011 sur 106 stations dans la lagune de Thau, où les...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00092/20355/18015.pdf
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Fifty years of ecological changes: Regime shifts and drivers in a coastal Mediterranean lagoon during oligotrophication ArchiMer
Derolez, Valerie; Malet, Nathalie; Fiandrino, Annie; Lagarde, Franck; Richard, Marion; Ouisse, Vincent; Bec, Beatrice; Aliaume, Catherine.
Thau lagoon is a large Mediterranean coastal lagoons and it supports traditional shellfish farming activities. It has been subject to eutrophication leading to major anoxic events associated with massive mortalities of shellfish stocks. Since the 1970s, improvements have been made to wastewater treatment systems, which have gradually led to oligotrophication of the lagoon. The aim of our study was to determine how the decrease in nutrient inputs resulted in major ecological changes in Thau lagoon, by analysing five decades of time-series (1970–2018) of observations on pelagic and benthic autotrophic communities. We were able to identify two periods during the oligotrophication process. Period 1 (1970–1992) was considered a eutrophic period, characterised...
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00629/74068/73521.pdf
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Gametogenesis, spawning behavior and larval abundance of the Pacific oyster Crassostrea gigas in the Thau lagoon: Evidence of an environment-dependent strategy ArchiMer
Ubertini, Martin; Lagarde, Franck; Mortreux, Serge; Le Gall, Patrik; Chiantella, Claude; Fiandrino, Annie; Bernard, Ismael; Pouvreau, Stephane; Roque D'Orbcastel, Emmanuelle.
The Thau lagoon, located at the South of France, provides 10% of the French pacific oyster Crassostrea gigas production. Despite this intensive shellfish production, the larval recruitment of this species within the Thau lagoon is still misunderstood and spat collection remains highly variable; as a consequence, shellfish farmers rely mostly on hatchery”s spat. The present study aimed to describe reproduction features of C. gigas within the Mediterranean, as well as to explore spawning triggers of this species. To achieve this goal, the reproduction cycle from gametogenesis to spawning was followed during two years in several locations within the Mediterranean Thau lagoon. The spawning behavior of C. gigas within the Thau lagoon appeared to be slightly...
Tipo: Text Palavras-chave: Mollusc physiology; Spawning triggers; Oysters.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00369/48032/48118.pdf
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Gametogenic cycle and reproductive effort of the tropical blacklip pearl oyster, Pinctada margaritifera (Bivalvia : Pteriidae), cultivated in Takapoto atoll (French Polynesia) ArchiMer
Pouvreau, Stephane; Gangnery, Aline; Tiapari, Jerome; Lagarde, Franck; Garnier, Matthieu; Bodoy, Alain.
The gametogenic cycle and the reproductive effort of the blacklip pearl oyster, Pinctada margaritifera, cultivated in Takapoto lagoon were studied for a 1-year period (March 1997-April 1998) by bimonthly observations of gonadal sections, dry tissue weights and gonadal index in a population of pearl oyster composed of three age-groups. pearl oysters attained sexual maturity in the end of their first year (height approximate to 40 mm), implying that P. margaritifera is a late-maturing species in comparison with other Pteriidae. This species was also confirmed to be a marked protandrous successive hermaphrodite in culture, with 100 % of males at first maturity and 75 % in older pearl oyster (height > 120 mm). The general pattern of gametogenic activity,...
Tipo: Text Palavras-chave: French Polynesia; Reproductive effort; Reproductive cycle; Tropical aquaculture; Gametogenesis; Blacklip pearl oyster; Polynésie française; Effort de reproduction; Cycle de reproduction; Aquaculture tropicale; Gamétogenèse; Huître perlière.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-1391.pdf
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Growth of the black-lip pearl oyster, Pinctada margaritifera, in suspended culture under hydrobiological conditions of Takapoto lagoon (French Polynesia) ArchiMer
Pouvreau, Stephane; Tiapari, Jerome; Gangnery, Aline; Lagarde, Franck; Garnier, Matthieu; Teissier, Hinano; Haumani, G; Buestel, Dominique; Bodoy, Alain.
Growth of the black-lip pearl oyster, Pinctada margaritifera var. cumingi, was studied for an annual cycle, from March 1997 to April 1998, in the lagoon of Takapoto atoll (Tuamotu archipelago, French Polynesia). Growth in shell and in tissue were measured every 15 days on three successive age groups of cultivated pearl oysters. At the same time, hydrobiological parameters (temperature, salinity, oxygen concentration, suspended particulate matter), known to have influence on bivalve growth, were followed each week during culture. No seasonal trend was observed in hydrobiological parameters, except for temperature which varied between 26 degrees C and 31 degrees C. The potential food for pearl oysters (particulate organic matter, POM, mg l(-1)) was slightly...
Tipo: Text Palavras-chave: Pearl culture; Matter in suspension; Biochemical composition; Length weight relations ship; Tropical lagoon; Growth; Pinctada margaritifera.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-381.pdf
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